ORDERING INFORMATION

DESCRIPTION

The Prestained Protein Ladder is a three-color protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute and add reducing agent before loading.

CONTENTS

Approximately 0.2 - 0.4 mg/ml of each protein in buffer (20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v) Glycerol.

• 3 µl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
• 2 - 3 µl per well for general Western transferring
• Apply more, for thicker (>1.5 mm) or larger gel
  

QUALITY CONTROL ASSAYS

MOLECULAR WEIGHT ESTIMATION (kDa)

Migration patterns of Prestained Protein-Ladder in different electrophoresis conditions are listed below:

Note:The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.

Stable at 25°C for 2 weeks.

Stable at 4°C for 3 months.

Store at -20°C for 24 months.

For research use only. Caution: Not intended for human or animal diagnostic or therapeutic uses.